Technician/Senior Technician

2 mesi fa

Milano, Italia Human Technopole Foundation A tempo pieno

APPLICATION CLOSING DATE:October 5th 2024 The Human Technopole Foundation is seeking a highly motivated Technician/Senior Technician (maternity cover) to join our National Facility for Genome Engineering and Disease Modeling (GEDI). Please note that this hiring is urgent. This facility is at the forefront of patient-specific disease modeling, focusing on scaling up innovative laboratory protocols into automated workflows. By integrating cutting-edge robotic and bioengineering platforms, we aim to standardize and benchmark these protocols through a multidisciplinary approach.

Working in close collaboration with other National Facilities and the Flow Cytometry and Electrophysiology support units, GEDI is dedicated to developing transformative technologies. These efforts are aligned with Human Technopole's vision of advancing high-definition biomedicine using state-of-the-art genome engineering techniques and sophisticated organoid systems at single-cell multiomic resolution.

The successful candidate will report to the Head of the Facility and will have the opportunity to work in a multidisciplinary environment to develop and support the execution of experimental activities in the following areas: Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs), followed by characterization and banking of the generated iPSCs.

Key tasks and responsibilities: Carry out, in synergy with the facility team, the experimental activities envisaged for the provision of Facility services in the field of pluripotent stem cell generation and maintenance. Support the team in the development of innovative protocols for the generation and maintenance of iPSCs. Support the head of the Facility in setting up core resources, participating in key aspects of general facility management and organization (e.g., managing inventories and stocks of samples, reagents, equipment and materials). Mandatory requirements: A master's degree in molecular biology or in a related field of the life sciences is required. At least 1 year of relevant work experience. Experience with human pluripotent stem cell lines. Experience and skills in performing repetitive and specialized laboratory experimental procedures. Experience following experimental protocols. Fluency in English – HT is an international research institute. Preferential requirements: A PhD in molecular biology, genetics, biochemistry, or in a related field of the life sciences. Experience with iPS cell reprogramming from somatic cells (e.g. PBMCs). Experience in molecular biology (DNA and RNA manipulation, cloning, sequencing, RT-PCR, single cell manipulation, FACS). Familiarity with 3D cell cultures (generation of organoids). Familiarity with basic molecular biology techniques such as qRT-PCR. Familiarity with Protein Biochemistry methods (immunoblotting and immunoprecipitation techniques, preferably also recombinant protein expression, purification and in vitro reactions), tissue processing and histology (preferably on mammalian brain). Organizational and social skills: Ability to manage competing priorities in a fast-paced environment and to work independently. Strong interpersonal skills demonstrated by the ability to lead and gain the assistance and cooperation of others in a team endeavor. Ability to build trust through operating with transparency and creating an open and positive environment. Ability to work in a multi-cultural, multi-ethnic environment with sensitivity and respect for diversity. Application Instructions: Please apply online sending:

A full CV. A motivation letter. Referee contacts. WHY HUMAN TECHNOPOLE? HT seeks for scientific excellence, we recruit the best scientific talents through international, open calls and meritocratic selection procedures. Our working environment is international, friendly and inclusive. Our scientists work together across disciplines on research topics of biomedical relevance, leveraging synergies between their diverse skillsets and methodological approaches. We believe that highly diverse teams yield the best and most innovative results. We engage in outward-facing scientific activities aimed at benefiting the national and international research community. Training is also at the heart of our activity, with initiatives and opportunities for our staff, including scientific courses, conferences and workshops. Main benefits: Welfare plans. Canteen service. Work-life balance provisions. Italian language training for foreigners. Maternity leave up to 1 year and other support for new parents. Possibility of flexible working hours. Support for relocation. Special consideration will be given to candidates who are part of the protected categories list, according to L. 68/99. Number of positions offered: 1

Contract offered: CCNL Chimico Farmaceutico, fixed-term - Employee level.

Salary range: Depending on candidate's seniority.

Tax benefits where applicable. The position is based in Milan.

"The Foundation reserves the right, at its sole discretion, to extend, suspend, modify, revoke, or cancel this job posting without giving rise to any rights or claims whatsoever in favor of the candidates; the Foundation reserves, however, the right not to proceed with the awarding of the above-described assignment due to the effect of supervening regulatory provisions and/or obstructive circumstances."

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