Technician Protein Biochemistry

3 settimane fa

Milano, Italia Human Technopole A tempo pieno

**APPLICATION CLOSING DATE: 20th of March 2024**

**Human Technopole (HT) **is an interdisciplinary life science research institute created and supported by the Italian Government to develop innovative strategies for improving human health. HT comprises five Centres: Neurogenomics, Computational Biology, Structural Biology, Genomics, and Health Data Science. The Centres work together to enable interdisciplinary research and create an open and collaborative environment that will help promote life science research nationally and internationally.

**About the lab**:The **Zerial Group** works on endocytosis - from its molecular mechanisms to implications in cell and tissue organisation. Its interests range from the assembly and functional characterisation of the endosomal fusion machinery to establishing hepatocyte cell polarity and liver tissue structure and function. The Group uses a unique interdisciplinary approach combining biochemical and biophysical methods with advanced light microscopy, cell biology and computer-aided three-dimensional (3D) tissue reconstruction to investigate the organisation and function of the mouse and human liver tissue.

The **Zerial Group** **is seeking one highly motivated technician with a solid background in protein biochemistry **to join its interdisciplinary team of molecular cell biologists, biochemists, biophysicists, and computer scientists.

**See previous work on the research topics of this call**:
The Rab5 effector FERRY links early endosomes with mRNA localization - ScienceDirect

A non-linear system patterns Rab5 GTPase on the membrane | eLife (elifesciences.org)

**Key tasks and responsibilities**

Specific tasks include:

- Investigate protein trafficking and compartmentalisation on the endosomal membrane.
- Protein purification from different sources.
- Design and execution of biochemical and in vitro reconstitution assays.
- Use specialised laboratory equipment to perform tests and analyse samples/data.
- Support, train, and assist in supervising group members with their work.
- Work closely with other technical staff members on general organisation and administrative duties for the organisation of the lab.
- Keep detailed, accurate and understandable records of experimental plans and work, and perform data analysis and statistical assessment.
- Participate in managing laboratory reagents, equipment, consumables, and stocks.

**Mandatory requirements**:

- Bachelor/Master’s degree in biology, biotechnology or a related discipline.
- Excellent expertise in molecular and biochemical techniques.
- Proficiency in designing and executing biochemical and in vitro reconstitution assays.
- At least three years work experience in an academic environment.
- Ability to execute complex protocols.
- Fluency in English.

**Preferred requirements**:
**Organizational and social skills**:

- Ability to work independently and re-prioritise tasks based on emerging needs and requests from other team members.
- Good organisational skills and ability to multitask.
- Strong problem-solving skills.
- Excellent communication skills.

**Application Instructions**

i) a CV
ii) a motivation letter in English
iii) names and contacts of at least 2 referees

**Why Human Technopole**
- HT seeks **scientific excellence**. We recruit the best scientific talents through international open calls.
- Our working environment is** international, friendly and inclusive**. Our scientists work together across disciplines on research topics of biomedical relevance, leveraging synergies between their diverse skill sets and methodological approaches.
- We believe that highly **diverse teams yield the best and most innovative results**.
- We engage in outward-facing scientific activities for the national and international research community. **Training** is also at the heart of our activity, with initiatives and opportunities for our staff, including scientific courses, conferences and workshops.

**Main benefits**
- Welfare plans.
- Canteen service.
- Work-life balance provisions.
- Italian language training for foreigners.
- Maternity leave up to 1 year and other support for new parents.
- Counseling.
- Flexible working hours.
- Remote working policy.
- Support for relocation.

**Number of positions offered**: 1
**Contract offered**: CCNL Chimico Farmaceutico, Fixed-term - 5 years - employee level.
The position is based in Milan

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