Internship: Antibody Design Intern

2 settimane fa

Siena, Italia GSK A tempo pieno

**Site Name**: Italy - Siena

**Posted Date**: Feb 27 2024
**Posting Title: Antibody Design Intern - Molecular Biology & Bacteriology, Italy - 2024**

**Help us get ahead of disease together with our Antigen & Antibody Design and Expression Team**

**Education required**: Master's degree in Molecular Biology, Biology, Biotechnology or similar field

**Other requirements**:
Hard skill:

- Good knowledge of molecular biology, cellular biology and immunology, and previous basic experience in molecular biology or microbiology laboratory.
- Knowledge of PCRs and cloning techniques
- Proficiency in English

Soft Skill:

- Ability to work in a multidisciplinary environment
- Ability to work in team and in a supervised environment
- Organizational skills
- Good communication skills
- Optimistic mindset
- Problem solving oriented

**Application deadline**: March 12th

**Duration**:6 Months

**Start date**: April 8th

**Type**: Extra-Curricular Internship

100% Presence

A GSK internship offers you the opportunity to kickstart your career - to take on a real role with genuine impact You’ll take on challenging, serious work on live projects or assignments. You’ll also get to learn from us, develop your skills and gain valuable experience for wherever the future takes you.

**What will you do?**:
**Context**

Intern will be in the Antigen & Antibody Design and Expression group, Molecular Biology & Bacteriology IT Funtion.

Within the monoclonal Antibodies (mAbs) discovery, our group has implemented antigen specific human and mouse monoclonal antibodies sequencing and throughput expression protocols, with the aim to further purify and functionally characterize antigen positive mAbs. Also, we are involved in protein expression as full length or antigen domains recombinant in _E. coli,_ to support vaccines discovery projects.

We need someone able to help us in mAbs and protein antigens genetic characterization and expression for GSK project requests.

The aims of this project are:

- Sequencing and production of antigen specific recombinant mAbs from human or mouse sources. V region of Heavy and Light Variable chains of single sorted B-cells are amplified and sequenced; detected V-region are inserted in recombinant expression plasmids for mAbs expression in mammalian cells Expi293;
- Support on the antigens protein expression in _E. coli_ system and protein expression evaluation;

Main expected result of this project is the increase of our abilities in sequencing, handling and express antigen specific mAbs and support different GSK projects for protein antigens production.

**Competencies the intern will develop thanks to the internship experience**

This internship will allow the intern to develop following experiences:

- Increase molecular biology, cellular biology microbiology and immunology knowledge;
- Gain experience in RT, PCRs and cloning techniques;
- Improve abilities in mammalian cells handlings and mAbs expression;
- Approach in-vitro assays for preliminary mAbs characterization;
- _ E. coli_ growth for and control of proteins expression;
- Seed storage in internal cell banking;
- Report writing.

**Let’s do this**

You’ll find hints, tips and guidance on our recruitment process on our website

**Why Us?**

GSK is a global biopharma company with a special purpose - to unite science, technology and talent to get ahead of disease together - so we can positively impact the health of billions of people and deliver stronger, more sustainable shareholder returns - as an organization where people can thrive. Getting ahead means preventing disease as well as treating it, and we aim to positively impact the health of 2.5 billion people by the end of 2030.

Our success absolutely depends on our people. While getting ahead of disease together is about our ambition for patients and shareholders, it’s also about making GSK a place where people can thrive. We want GSK to be a workplace where everyone can feel a sense of belonging and thrive as set out in our Equal and Inclusive Treatment of Employees policy. We’re committed to being more proactive at all levels so that our workforce reflects the communities we work and hire in, and our GSK leadership reflects our GSK workforce.

GSK is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive equal consideration for employment without regard to race, color, national origin, religion, sex, pregnancy, marital status, sexual orientation, gender identity/expression, age, disability, genetic information, military service, covered/protected veteran status or any other federal, state or local protected class.

**Important notice to Employment businesses/ Agencies**

Please note that if you are a US Licensed Healthcare Professional or Healthcare Professional as defined by the laws of the state issuing your license, GSK may be required to capture and report expenses GSK incurs, on your behalf, in the event you are afforded an interview f

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