Development of Biochemical Assays

3 settimane fa

Bresso, Italia AXXAM SPA A tempo pieno

Axxam is a leading provider of integrated discovery services for the entire Life Sciences industries such as Pharmaceutical, Crop protection, Animal health, Cosmetics and Nutrition. We have a strong expertise across a broad range of drug discovery areas, including assay development, high-throughput screening, and hit-to-lead programmes. Axxam is also engaged in developing novel innovative therapies for diseases with a high unmet medical need. An open and dynamic working environment supported by an industrialized organization ensures high-quality results.

**Development of Biochemical Assays - Enzymologist**:
Axxam is opening a position for a scientist, to join the group of Biochemistry. The position requires strong scientific and technical expertise and proven track records of development of biochemical assays suitable to be configured for high-throughput screening and characterisation of enzyme kinetics and/or protein interaction and their modulation, as well as an excellent understanding of the underlying mechanisms.

The experienced enzymologist will be responsible to devise the project strategy to accomplish proof of principle studies by scouting different assay systems and to develop high-throughput homogeneous assays in miniaturized format with fluorescent and luminescent readout systems. The assays are intended to measure the catalytic activity of target enzymes or the interaction between proteins, or between proteins and biologically relevant macromolecules. The assays will be applied in screening campaigns and to assess potency, selectivity, and mechanism of action of small molecule modulators of the target proteins. Besides, the successful scientist will be occasionally in charge of the recombinant expression of the target proteins in insect cells with the baculovirus system and/or in high-density mammalian cells cultured in suspension, and their purification through different chromatographic techniques. The position requires extensive collaboration with colleagues and effective communication skills in English with internal and external international partners.

**Core job responsibilities**:
Strategy design, development, and optimization of biochemical assays for high-throughput screening in 384 well/plate format for the detection of the catalytic activity of the target enzymes and/or of the interaction of the target proteins with partner proteins, peptides, nucleic acids, and other molecular effectors
Detailed biochemical and enzymatic characterization of the assays, to include determination of kinetic parameters and/or affinity parameters, together with fulfilment of key quality criteria for the adaptation to automation for HTS
Characterization of the potency, selectivity, and mechanism of action of reference modulators and of small libraries of compounds
Recombinant expression of the target proteins in eukaryotic expression hosts i.e., insect cells with the baculovirus system and/or mammalian cells cultured in suspension at high scale, their analytical and preparative chromatographic purification by FPLC, and their biochemical characterisation
Design of the experiments in miniaturized and parallelized format, analysis of the data, generation of suitable graphical representations, interpretation of the results, identification of possible inconsistencies and liabilities, and proposal of action steps and contingency plans
Preparation of regular bi-weekly updates in PowerPoint format, with presentation of the data to international clients in face-to-face meetings and teleconferences, with active discussion of the results and their perspective implications for the progression of the project
Initiative-taking support to colleagues in collaborative projects, by ensuring reliability, accuracy, robustness, and confidentiality for data generated in cooperation with external clients
Keeping pace with the technological and scientific developments and innovation in the fields of interest.

**Required knowledge and skills**:
Degree in Biology, Pharmacological Sciences, or related Life Sciences Academic Faculties with at least five-year traceable hands-on experimental research in Enzymology/Biochemistry, preferably spent in an industrial environment
Significant expertise in enzyme kinetics and inhibition/activation applied to drug discovery projects, or alternatively in the determination of the binding affinity parameters for protein interaction systems using state-of-the-art methods
Expertise in one or more of the following readout systems is essential: fluorescence intensity, FRET, fluorescence polarization, TR-FRET, and luminescence. Prior experience in biophysical techniques such as NMR, X-ray crystallography, MS-LC, SPR, TSA, MST, ITC, DSF, is highly regarded
Prior experience with miniaturized and parallelized 96 or 384 well/plate assay formats and lab automation systems
Experience in expression of recombinant proteins produced with eukaryotic host cells i.e., insect cells with the baculovirus system and

  • Cell Biology Scientist Experienced in Ipsc

    1 settimana fa

    Bresso, Italia AXXAM SPA A tempo pieno

    Axxam is a leading provider of integrated discovery services for the entire Life Sciences industries such as Pharmaceutical, Crop protection, Animal health, Cosmetics and Nutrition. We have a strong expertise across a broad range of discovery disciplines and innovative technologies including: assay development, compound management, High Throughput Screening,...

  • Associate Clinical Director

    2 settimane fa

    Bresso, Italia Experis A tempo pieno

    For our client, a multinational late-stage biotech company, we are seeking an experienced clinical professional to help us lead in the clinical aspects of project management activities in close collaboration with her/his counterparts in CMC and regulatory. **Essential duties and responsibilities**: - Manage clinical data (tables, listings, SAP, IB, PK,...

  • Analytical Methods Development Technician

    1 mese fa

    Bresso, Italia AGC Biologics A tempo pieno

    Our purpose is to bring hope to life by enabling life-changing therapies for patients around the globe, creating a healthier and happier tomorrow. Our mission is to work side by side with our customers in order to improve patients’ lives by bringing new biopharmaceuticals to market. Citofluorimetria - Our culture at AGC Biologics is defined by the six...

  • Cell Process Development Technician

    2 mesi fa

    Bresso, Italia AGC Biologics A tempo pieno

    Our purpose is to bring hope to life by enabling life-changing therapies for patients around the globe, creating a healthier and happier tomorrow. Our mission is to work side by side with our customers in order to improve patients’ lives by bringing new biopharmaceuticals to market. Cell process - Our culture at AGC Biologics is defined by the six core...

  • Human Resources Generalist

    2 settimane fa

    Bresso, Italia Novelis Bresso Manufacturing Facility A tempo pieno

    Position Overview: Purpose of the role is to manage, support and improve HR-related process. Contribute to act as point of contact for employees, ensure deployment of HR projects. Provides also proactive HR Administration support. Responsibilities & Qualifications: Responsabilities - Payroll: Provide dedicated support to multiple client with specific focus...

  • Human Resources Generalist

    2 settimane fa

    Bresso, Italia Novelis A tempo pieno

    Panoramica della posizione: Purpose of the role is to manage, support and improve HR-related process. Contribute to act as point of contact for employees, ensure deployment of HR projects. Provides also proactive HR Administration support. Responsabilità e qualifiche: Responsabilities - Payroll: Provide dedicated support to multiple client with specific...

  • Upstream Vector Development Technician

    2 mesi fa

    Bresso, Italia AGC Biologics A tempo pieno

    Our purpose is to bring hope to life by enabling life-changing therapies for patients around the globe, creating a healthier and happier tomorrow. Our mission is to work side by side with our customers in order to improve patients’ lives by bringing new biopharmaceuticals to market. Attività principali: - Contribuire allo sviluppo, ottimizzazione ed...

  • Downstream Vector Development Technician

    2 mesi fa

    Bresso, Italia AGC Biologics A tempo pieno

    Our purpose is to bring hope to life by enabling life-changing therapies for patients around the globe, creating a healthier and happier tomorrow. Our mission is to work side by side with our customers in order to improve patients’ lives by bringing new biopharmaceuticals to market. Attività Principali: - Contribuire allo sviluppo, ottimizzazione ed...

  • Analytical Methods Development Technician

    4 mesi fa

    Bresso, Italia AGC Biologics A tempo pieno

    Our purpose is to bring hope to life by enabling life-changing therapies for patients around the globe, creating a healthier and happier tomorrow. Our mission is to work side by side with our customers in order to improve patients’ lives by bringing new biopharmaceuticals to market. Attività principali: - Esecuzione di metodi analitici per la...

  • Controller - Internship

    7 mesi fa

    Bresso, Italia AXXAM SPA A tempo pieno

    Axxam is a leading provider of integrated discovery services for the entire Life Sciences industries such as Pharmaceutical, Crop protection, Animal health, Cosmetics and Nutrition. We have world-class expertise across a broad range of discovery disciplines and innovative technologies including assay development, compound management, high throughput...

  • Junior Controller

    7 mesi fa

    Bresso, Italia AXXAM SPA A tempo pieno

    Axxam is a leading provider of integrated discovery services for the entire Life Sciences industries such as Pharmaceutical, Crop protection, Animal health, Cosmetics and Nutrition. We have world-class expertise across a broad range of discovery disciplines and innovative technologies including assay development, compound management, high throughput...

  • Junior Controller

    7 mesi fa

    Bresso, Italia AXXAM SPA A tempo pieno

    Axxam is a leading provider of integrated discovery services for the entire Life Sciences industries such as Pharmaceutical, Crop protection, Animal health, Cosmetics and Nutrition. We have world-class expertise across a broad range of discovery disciplines and innovative technologies including assay development, compound management, high throughput...

  • Junior Controller

    7 mesi fa

    Bresso, Italia AXXAM SPA A tempo pieno

    Axxam is a leading provider of integrated discovery services for the entire Life Sciences industries such as Pharmaceutical, Crop protection, Animal health, Cosmetics and Nutrition. We have world-class expertise across a broad range of discovery disciplines and innovative technologies including assay development, compound management, high throughput...

  • Safety Associate

    6 giorni fa

    Bresso, Italia AGC Biologics A tempo pieno

    Our purpose is to bring hope to life by enabling life-changing therapies for patients around the globe, creating a healthier and happier tomorrow. Our mission is to work side by side with our customers in order to improve patients’ lives by bringing new biopharmaceuticals to market.Requisiti:Our culture at AGC Biologics is defined by the six core values:...

  • Regulatory Affairs Specialist

    2 ore fa

    Bresso, Italia In Job S.p.A. A tempo pieno

    In Job S.p.A. – Milan's Career Center is hiring a Regulatory Affairs Specialist for a lead company in the pharmaceutical industry.KEY RESPONSIBILITIESSupport product development and manage registration and maintenance of new and/or existing products worldwide;Assist in updating and maintaining the Regulatory Information Management System (RIMS);Contribute...

  • Regulatory Affairs Specialist

    2 ore fa

    Bresso, Italia In Job S.p.A. A tempo pieno

    In Job S.p.A. – Milan's Career Center is hiring a Regulatory Affairs Specialist for a lead company in the pharmaceutical industry.KEY RESPONSIBILITIESSupport product development and manage registration and maintenance of new and/or existing products worldwide;Assist in updating and maintaining the Regulatory Information Management System...

  • Msat Compliance Associate

    7 mesi fa

    Bresso, Italia AGC Biologics A tempo pieno

    Our purpose is to bring hope to life by enabling life-changing therapies for patients around the globe, creating a healthier and happier tomorrow. Our mission is to work side by side with our customers in order to improve patients’ lives by bringing new biopharmaceuticals to market. Main activities: - Support MSAT and Development units in activities...

  • Scientist / Cell Biology

    3 giorni fa

    Bresso, Italia AXXAM SPA A tempo pieno

    Axxam is a leading provider of integrated discovery services for the entire Life Sciences industries such as Pharmaceutical, Crop protection, Animal health, Cosmetics, Nutrition, and Flavour & Fragrances. We have a strong expertise across a broad range of discovery disciplines and innovative technologies including assay development, compound management, HTS,...

  • Grant Office Specialist

    7 mesi fa

    Bresso, Italia AXXAM SPA A tempo pieno

    Axxam is a leading provider of integrated discovery services for the entire Life Sciences industries such as Pharmaceutical, Crop protection, Animal health, Cosmetics and Nutrition. We have world-class expertise across a broad range of discovery disciplines and innovative technologies including assay development, compound management, high throughput...

  • GMP Manufacturing Technician

    1 settimana fa

    Bresso, Italia AGC Biologics A tempo pieno

    GMP Manufacturing Technician - Cell FactoryApply locations: Bresso, ItalyTime type: Full timePosted on: Posted 30+ Days AgoOur purpose is to bring hope to life by enabling life-changing therapies for patients around the globe, creating a healthier and happier tomorrow. Our mission is to work side by side with our customers in order to improve patients’...