Proteomics Specialist

Build the science that shapes the future of human health. Application closing date: 08 November 2026 Join a place where ambitious science thrives Human Technopole (Milan) is a rapidly expanding life science institute where international researchers and cutting-edge technologies converge to accelerate biomedical discovery. Our mission is to transform bold scientific ideas into advances that improve human health. Within this mission, The Structural Proteomics Unit studies protein structure and interactions using advanced mass spectrometry–based proteomics, with a strong focus on crosslinking MS and high-throughput workflows, including single-cell proteomics. It operates state-of-the-art instrumentation and advanced data analysis pipelines within the multidisciplinary ecosystem of Human Technopole. the Proteomics Specialist will report to the Unit Manager and collaborate closely with National Facilities and research groups. The focus of the Proteomics Specialist will be to support high throughput proteomics of cell lines, organoids, tissues and biofluids, together with the other members of the team. Your mission

• Develop and optimize advanced proteomics workflows Establish robust protocols for spatial/visual, plasma and clinical proteomics, including bottom-up sample preparation pipelines and peptide enrichment strategies.
• Operate and maintain mass spectrometry platforms Perform instrument calibration, cleaning, basic maintenance, and support automation of peptide extraction, digestion, and cleanup for diverse sample types.
• Manage large-scale proteomics studies Oversee processing and data acquisition for high-throughput projects involving hundreds to thousands of samples, ensuring quality and reproducibility.
• Analyze data and support facility users Deliver and explain results to users, contribute to experimental design and project planning, provide technical support during projects, and train internal and external researchers.
• Contribute to the growth of the Unit Support resource planning, introduce new protocols and applications, collaborate with vendors and service engineers, mentor junior staff, and participate in facility reviews and research initiatives.
• Support facility operations and quality assurance Manage facility digital platforms and software, support procurement and inventory monitoring, perform advanced troubleshooting of protocols and technical issues, contribute to the development and implementation of Standard Operating Procedures (SOPs) and quality assurance parameters for reporting and auditing.
• Support facility operations and quality assurance Manage facility software, support procurement and inventory monitoring, perform advanced troubleshooting of protocols and technical issues.

Grow Your Skills You will enhance your scientific and professional skills by working with:

• cutting-edge proteomics technologies, including state-of-the-art mass spectrometry platforms, automation, and advanced workflows for single-cell, spatial, and clinical proteomics.
• complex experimental design and large-scale data generation, ensuring high-quality, reproducible results in a high-throughput facility environment.
• multidisciplinary collaboration and facility operations, developing expertise in project coordination, user support, technology development, and mentoring within a leading research infrastructure.

Human Technopole supports career development through training, mentoring and dedicated learning opportunities. Essential What you’ll bring

• PhD in a relevant subject (e.g. biology, biotechnology, biochemistry, chemistry) to be obtained within the deadline of the call.
• At least 5 years of prior experience with bottom-up proteomics by mass spectrometry (this can include PhD years) including sample preparation.
• Fluency in English – HT is an international research institute.
• Familiarity with proteomics search engines (MaxQuant, FragPipe, Dia-NN, Spectronaut or similar).
• One or more of the following: experience with plasma proteomics, spatial/tissue proteomics, clinical proteomics, single cell proteomics
• Experience with implementing mass spec/proteomics methods at the spectrometer.
• Familiarity with operating and troubleshooting LC-MS platforms.

Preferred Any one of the following:

• Experience with DIA for global untargeted proteomics
• Familiarity with Astral, Hybrid or Tribrid Mass Spectrometers.
• Experience in working with large cohorts and/or patient sample sets, including benchmarking and QC.
• Experience in standardising workflows and/or developing targeted assays.
• Experience with machine learning in proteomics data analysis.
• Experience with