Research Assistant Position
1 settimana fa
A Research Assistant position is immediately available at San Raffaele Telethon Institute for Gene Therapy (SR-Tiget) Mechanisms of Peripheral Tolerance Unit, Milan, Italy. We are looking for a highly motivated Research Assistant with a strong background in immunological assays,and expertise in experimental animal models to work as part of an enthusiastic and dynamic research teaminvestigating multiple immunological aspects associated adverse immune responses in the context of geneand cell therapies for inherited genetic diseases, and autoimmune diseases.
**Main activities**:
Assays to investigate antigen (Ag)-specific cellular responses (primary cells from several disease models);
Establishment of Ag-specific T cell lines;
Differentiation of dendritic cells (DC) and their use for Ag presentation in vitro and in vivo experiments;
LV mediated modification of DC, B and T cells for in vitro and in vivo experiments;
Cytokine quantification (Multiplex assay-Luminex);
Determination of Ag-specific antibodies (Abs) titer (ELISA);
Basic Molecular biology and biochemistry (PCR, cloning, western blots, enzymatic activity test);
Flow cytometry (sample preparation/acquisition/analysis, FACS sorting);
Development and optimization of new assays;
Vector production.
**Requirements**:
Biomedical degree or PhD with strong lab experience;
High accuracy, reliability, precision;
Experience in T cell functional immune assays;
Experience in B cell functional immune assays will be a plus in the selection;
Experience in animal handling;
High motivation to work in an international, fast-paced environment;
Excellent organization skills;
Friendly, collaborative, pro-active mindset;
Good oral and written English skills.
**We offer**:
One-year fixed-term contract (assunzione a tempo determinato), renewable;
Competitive salary, based on qualification and experience;
A dynamic lab environment and the chance to make important contribution to biomedical research.
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