Tissue Processing Postdoc

3 years position

About the job/team

A collaboration between EMBL Rome, EMBL Hamburg and EMBL Heidelberg has been awarded funding from CZI to link the detailed cellular morphology of a tissue with its repertoire of macromolecules: High Throughput X-ray Tomography to Target Transcriptomics (HiTT2T).

The Light Imaging Facility at EMBL Rome have developed spatial-omics technologies that permit multiplex RNA detection in thin tissue sections. Separately, EMBL Hamburg has recently established HiTT (High Throughput Tomography) - an X-ray imaging workflow which offers a fast and high-throughput approach to 3D structural imaging and virtual histology characterisation of entire tissue samples within minutes. The ability to target the 2D-omics data production based on the 3D X-ray data has the potential to revolutionise our understanding of disease. With this proposal, we will move beyond the current state-of-the-art of the methodologies and go to high throughput volumetric tissue analysis and transcriptomics via the development of HiTT2T - High Throughput Tomography to Targeted Transcriptomics. Using the information obtained via HiTT2T and the power of computational analysis presents the possibility of predicting and/or preventing disease with a greater degree of accuracy in the future.

Your role

The fellow is expected to develop tissue processing methods for imaging-based spatial -omics. This position has a strong focus on wet-lab work and experimental protocol development to improve tissue processing methods for -omics. The fellow will work within the Light Imaging Facility at EMBL Rome.

You have:

- Knowledge in molecular biology
- Experience in tissue, whole-organ or cell sample preparation methods (from basic to IF, RNA visualization up to clearing techniques and expansion microscopy).
- General knowledge in microscopy and image analysis
- Excellent manual dexterity and organizational skills.
- Pro-active attitude and hands-on approach to problem solving.
- Excellent communication, teamwork and interpersonal skills with highest ethical standards.
- Fluency in written and spoken English.

You may have:

- Knowledge in super-resolution microscopy, fluidics or microscopy automation,
- Knowledge in bioinformatics in particular oligo design for RNA or DNA detection as well as analysis of -omics methods.
- Knowledge on multiplex imaging, spatial transcriptomics or bioinformatic analysis of -omics data would be advantageous.
- Knowledge in computer vision and expertise in a programming language such as Python, R or Matlab

Professional development support

The EMBL Fellows’ Career Service provides support and guidance to predoctoral and postdoctoral fellows across all six EMBL’s sites.

Working with a dedicated Careers Advisor, this invaluable service will help you to take informed decisions about your career planning both in the short and longer term. Whether your main interest is pursuing a career path in academia, exploring opportunities in industry or exploring an independent venture, the EMBL Fellows’ Career Service with provide you with a portfolio of activities and resources to help you.

Why join us

Join a culture of innovation
We are located on the Wellcome Genome Campus, alongside other prominent research and biotech organisations, and surrounded by beautiful Cambridgeshire countryside. This is a highly collaborative and inclusive community where our employees enjoy a relaxed atmosphere. We are committed to ensuring our employees feel valued, supported and empowered to reach their professional potential.

Enjoy lots of employee benefits:
- Financial incentives: Monthly family and child allowances, generous stipend reviewed yearly, pension scheme, death benefit and unemployment insurances- Flexible working arrangements including hybrid working patterns- Private medical insurance for you and your immediate family- Generous time off: 30 days annual leave per year in addition to public holidays- Campus life: Free shuttle bus to and from work (Heidelberg), on-site library, subsidised on-site gym and cafeteria, casual dress code, extensive sports and social club activities (on campus and remotely)- Family benefits: Kindergarten (Heidelberg), 10 days of child sick leave, paid maternity & parental leave, holiday clubs on campus and monthly family and child allowances

What else you need to know-
- international applicants

.-
- Job location: All our fellowships are based on-site (for at least part of each week). If you are living overseas, you will receive a generous relocation package to support you.-
- here Closing Date

28/02/2025