Mrna Scientist

7 mesi fa

Monza, Italia Thermo Fisher Scientific A tempo pieno

Thermo Fisher Scientific Inc. is the world leader in serving science, with annual revenue of more than $40 billion. Our Mission is to enable our customers to make the world healthier, cleaner and safer. Whether our customers are accelerating life sciences research, solving complex analytical challenges, increasing productivity in their laboratories, improving patient health through diagnostics or the development and manufacture of life-changing therapies, we are here to support them. Our global team delivers an unrivaled combination of innovative technologies, purchasing convenience and pharmaceutical services through our industry-leading brands, including Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific, Unity Lab Services, Patheon and PPD.- Responsibilities:
- Develop and implement scalable mRNA purification strategies, to generate highly pure material.
- Design, develop, and implement workflows for high-throughput mRNA production.
- Use a data-driven approach to progress development deliverables.
- Develop robust and scalable downstream processes at the laboratory scale for the purification of nucleic acids with chromatography.
- Conduct troubleshooting process performance issues.
- Support scale-up of upstream/downstream processes and technology transfer to the cGMP manufacturing and quality control (QC) groups for clinical material production/analysis.
- Perform routine bench work under limited supervision, effectively prioritize responsibilities and complete assignments on time.
- Discuss internally and with customers the progress of the activities proposing technical solutions.
- Able to analyze, interpret, and present results to project teams and write technical reports.
- Maintaining domain expertise in new technologies and scientific literature to constantly drive innovation.
- Train junior scientists as needed.
- Qualifications:
- Requirements:
- Demonstrated experience with standard analytical equipment (plate reader, nanodrop, spectrophotometer, PCR, qPCR, electrophoresis) and knowledge of molecular biology, biochemistry, and biophysics protocols is required.
- Strong technical expertise in (two or more): in vitro transcription (IVT) synthesis, molecular cloning, purification methods by liquid chromatography (LC), capillary gel electrophoresis (CGE), high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS).
- Expertise in upstream processing, including experience with bioreactors at small, intermediate, and large scales; development of scale-up and scale-down models and bioreactions optimization.
- Experience in process development for therapeutics with standard downstream purification techniques, including depth filtration, FPLC and Tangential Flow Filtration (TFF).
- Familiarity with the process development of mRNA products would be a plus.
- Experience with GLP and GMP compliance and manufacturing preferred.
- Experience writing content for documentation, including SOPs, work instructions, technical protocols and reports, and technical presentations.
- A high level of commitment and a track record of quality work, with attention to detail.
- Capable of communicating the work clearly among the team.
- Ability to multi-task and prioritize to meet important deadlines.
- Ability to respond quickly to rapidly changing priorities.
- A self-starter and team player.
- Excellent written and spoken English

Thermo Fisher Scientific is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, creed, religion, color, national or ethnic origin, citizenship, sex, sexual orientation, gender identity and expression, genetic information, veteran status, age or disability status.
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