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Senior Research Technician

2 mesi fa


Milano, Lombardia, Italia Human Technopole A tempo pieno
About the Role

We are seeking a highly motivated and experienced Senior Research Technician to join our team at Human Technopole. As a key member of our Neurogenomics Research Centre, you will be responsible for providing laboratory support for projects involving human stem cell and organoids culture, as well as cutting-edge molecular biology and neuroscience technologies.

Key Responsibilities
  • Conduct experiments in the area of stem cell neurobiology, combining cutting-edge methods from molecular biology, genome editing, biochemistry, cell biology, and organoids, with a strong emphasis on advanced human cell culture pipelines, single-cell and spatial genomics approaches, and advanced in vivo modeling assays.
  • Optimize and scale up pipelines to perform cell culture experiments in high throughput and/or high content.
  • Generate and optimize new tools and protocols.
  • Generate reliable data for publication in the projects to which you are assigned and record all data and results in specified forms (with Electronic Lab Notebooks).
  • Provide technical support and supervision to other laboratory members, including introduction and teaching of new laboratory techniques and support on the use of lab equipment to lab members.
  • Liaise with the Centre's Lab Manager and other staff to write risk assessments and ensure that they are regularly updated.
  • Maintain cell lines generated and banked in the lab and ensure that all tissue culture users work according to health and safety guidelines and regulations.
  • Participate in key aspects of general lab management and organization (e.g., managing inventories and stocks of samples, reagents, equipment, and materials), in coordination with HT facilities and service units.
Requirements
  • MSc in biological or biomedical disciplines.
  • At least 5 years of proven experience as a Lab Technician.
  • Track record in the following areas: Advanced cell culture (preferably with human pluripotent stem cell lines, organoids, and primary neural cell cultures, including gene editing); Molecular Biology (DNA and RNA manipulation, cloning, sequencing, RT-PCR, and library preparation for next-generation sequencing applications), Protein Biochemistry (immunoblotting and immunoprecipitation techniques), Histology and microscopy (including tissue processing techniques, clearing, dissociation, and tissue processing for single-cell and spatial genomics, stainings, advanced microscopy).
  • Fluency in English – HT is an international research institute.
Preferred Requirements
  • Experience in high-throughput screening and/or high-content screening as well as with in vivo models will be considered a highly relevant added value.
Organizational and Social Skills
  • Ability to manage competing priorities in a fast-paced environment and to work independently.
  • Strong interpersonal skills demonstrated by the ability to lead and gain the assistance and cooperation of others in a team endeavor.
  • Ability to build trust through operating with transparency and creating an open and positive environment.
  • Ability to work in a multi-cultural, multi-ethnic environment with sensitivity and respect for diversity.
How to Apply

Please submit your application, including a CV, motivation letter in English, and names and contacts of 2 referees, through our online application system.